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Supp. 1 The mouse cell line GL261 and RCAS-PDGFb murine tumor model used in the present study contains a population of CD133+ cells with increased tumorigenic potential, which is lost after differentiation. (A) The percentage of CD133+ cells in neurosphere GL261 cells is 30,1% while in adhesive GL261 it is less than 1%. The mouse astrocytoma cell line GL261 contained a population of CD133+ cells, which could be purified by FACS with respective species-specific antibodies. The respective immunoglobulin isotypes (isotype control) served as controls. (B) RCAS-PDGFb murine tumor model contained a population of CD133+ cells, which could be purified by FACS with respective speciesspecific antibodies.The respective immunoglobulin isotypes (isotype control) served as controls. 30,1%

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تاریخ انتشار 2016